In this podcast episode, we delve into the challenges and advancements in quantifying microorganisms in absolute numbers, a critical aspect of quality control and microbiology research. From using techniques like qPCR and flow cytometry to addressing the limitations of current methods, experts share their insights on improving accuracy in microbiome analysis. Additionally, we discuss the importance of protocol standardization and its impact on developing more effective probiotic products. A must-listen for science and biotechnology enthusiasts! 🧪✨